Performance Characteristics of Nested Polymerase Chain Reaction vs Real-Time Polymerase Chain Reaction Methods for Detecting Mycobacterium tuberculosis Complex in Paraffin-Embedded Human Tissues

Quantitative Real-time Polymerase Chain Reaction (qRT-PCR). Fatemeh Mehrpooyan*

Detection of Nocardia Asteroides Complex in Clinical Isolates by Real-Time Polymerase Chain Reaction

Background and Aims: Nocardia asteroides complex is the most common cause of infectious diseases due to nocardiosis. Interspecies differentiation of Nocardia genera is essential for prognosis and timely proper treatment, as well as for epidemiological studies. Since each genus has its own antibiotic resistance, precise careful diagnosis is of prime importance. As compared to biochemical and phe...

Detection of Leishmania DNA in paraffin embedded specimens of chronic lupoid leishmaniasis using polymerase chain reaction

Background: Chronic lupoid leishmaniasis (CLL) is a chronicform of cutaneous leishmaniasis that is usually resistant to antileishmania agents and leishmania is not seen in the histologicsections. We used polymerase chain reaction (PCR) to detectleishmania DNA in CLL specimens.Method: This descriptive cross sectional study was done on20 paraffin embedded specimens of CLL cases referred to theder...

Polymerase Chain Reaction for the Diagnosis of Tuberculosis

Dear Editor-in-Chief We read with interest the study by Khazaei et al. (1) in which the authors have nicely concluded that PCR is more sensitive test than Ziehl-Neelsen staining and histo-pathological examination for the diagnosis of tuberculosis (TB). They have rightly pointed to use PCR, selectively, in acidfast bacilli negative paucibacillary forms of TB. However, we intend to highlight few...

Real-time polymerase chain reaction.

Real-time PCR is the state-of-the-art technique to quantify nucleic acids for mutation detection, genotyping and chimerism analysis. Since its development in the 1990s, many different assay formats have been developed and the number of real-time PCR machines of different design is continuously increasing. This review provides a survey of the instruments and assay formats available and discusses...